Isoelectric focusing technology quantifies protein signaling in 25 cells

Isoelectric focusing technology quantifies protein signaling in 25 cells.

A previously undescribed isoelectric focusing technology allows cell signaling to be quantitatively assessed in <25 cells. High-resolution capillary isoelectric focusing allows isoforms and individual phosphorylation forms to be resolved, often to baseline, in a 400-nl capillary. Key to the method is photochemical capture of the resolved protein forms. Once immobilized, the proteins can be prob...

Isoelectric focusing.

Capillary Isoelectric Focusing

Beale SC (1998) Capillary electrophoresis. Analytical Chemistry 70: 279. (This journal prints every two years a review on capillary electrophoresis. The article is written by an expert in the Reld and covers more or less all developments in CE including CGE of the preceding years.) Grossmann PD and Colburn JC (eds) (1996) Capillary Electrophoresis: Theory and Practice. San Diego: Academic Press...

Isoelectric focusing in a drop.

A novel approach to molecular separations is investigated using a technique termed droplet-based isoelectric focusing. Drops are manipulated discretely on a superhydrophobic surface, subjected to low voltages for isoelectric focusing, and split-resulting in a preparative separation. A universal indicator dye demonstrates the generation of stable, reversible pH gradients (3-10) in ampholyte buff...

Development of Isoelectric Focusing Techniques for Protein Analyses

Isoelectric focusing (IEF) is a powerful approach in separations of zwitterionic substances such as proteins, peptides and amino acids. It is important in proteomic research. Generally, in IEF, carrier ampholytes (CAs) are necessary to establish a stable pH gradient. However, CAs also bring attendant problems such as a decrease in detection sensitivity and suppression of ionization of analytes ...